ABSTRACT
ObjectiveTo observe the effection of different interventions of group B streptococcus (GBS) infection in gestation period.MethodsOne hundred and seventeen cases with GBS infection were obtained from 1885 pregnant women,who were got routine prenatal examination at 34 to 37 weeks of pregnancy,and drug seeitivity test of secretions which were taken from under paragraph 1/3 of vagina,and divided into treatment group (91 cases) and untreatment group (26 cases).The treatment group was divided into treatment group one (47 cases) and treatment group two (44 cases).Treatment group one was treated with oral antibiotics for 7 days after diagnosis,treatment group one and two were treated with postpartum antibiotics intravenous infusion once every 4 hours in labor.Comparison of maternal and fetal outcomes.ResultsThe GBS infection rate was 6.2％ (117/1885).The morbidity of premature delivery,premature rupture of membrane and neonatal infection of treatment group [ 5.5％ ( 5/91 ),13.2％ ( 12/91 ),5.5％ (5/91 ) ]were lower than those of untreatment group [ 19.2％(5/26),30.8％(8/26),23.1％(6/26) ](P ＜ 0.05).The morbidity of premature dehvery and premature rupture of membrane of treatment group one[ 0,6.4％ (3/47)]were lower than those of treatment group two[ 11.4％ (5/44),20.5％ (9/44)](P ＜ 0.05).Conclusion Anti-GBS treatment can improve the outcomes of mothers and infants,especially early anti-GBS treatment during the period of pregnancy.
ABSTRACT
To explore the anti-tumor effect of immunotherapy with recombinant protein vaccine based on FGFR-1 of chicken (cFR-1) in a mouse Meth A fibrosarcoma model, tumor volume and survival rate of the mice were observed at a 3-day interval. Microvessel density (MVD) was detected by immunohistochemistry. Auto-antibodies against self-FGFR-l were detected by Western blotting and ELISA, respectively. The anti-FGFR-1 antibody-producing B cells (APBCs) were detected by enzyme-linked immunospot (ELISPOT) assay. Eighteen days after inoculation of tumor cells, the tumor volume was significantly smaller in cFR-l-immunized group than in mouse FGFR-1 (mFR-1) immunized group and normal saline (NS) control group (P＜0.05), and the survival time was significantly longer in cFR-l-immunized group than in the control groups (P＜0.01). MVD was significantly lower in cFR-l-immunized group than in mFR-l-immunized group and NS group (16.8 ±5.6 vs 64.6±1.8and 59.6±8.7, P＜0.01). Antibodies against self-FGFR-1 were found in mFR-l-immunized group, the major antibody subclasses were IgG1 and IgG2b. Compared with the two control groups, the numbers of APBCs in cFR-l-immunized group were significantly increased (P＜0.01) These results demonstrated that the cFR-1-related anti-angiogenesis protein vaccine could induce the production of auto-antibodies against self-FGFR-1, which futher inhibit angiogenesis and growth of solid tumor.
ABSTRACT
BACKGROUND: The essential component of Plumbago zeylanica Linn is plumbagin, which plays a role in anti-leukemia, antibiosis, antifertility, as well as cardiovascular. OBJECTIVE: To investigate the effects of Plumbago zeylanica Linn on the healing process of experimental rabbit fracture. DESIGN, TIME AND SETTING: Randomized control experiment of animal was performed at the Department of Pharmacology, Guilin Medical College between December 2006 and February 2007. MATERIALS: Eight New Zealand rabbits, 80 to 85-day-old, weighing 2.1-2.5 kg, half male and half female. METHODS: Eight rabbits were randomly divided into the control and experimental groups, with 4 animals in each group. Under sterile conditions, the experimental bone fracture model of rabbit was created by making a longitudinal incision in the medial of the middle portion of right foreleg, cutting off a point-foot radius with bone clamp, and suturing the incision. The rabbits in the experimental group were treated externally with the extraction of Plumbago zeylanica Linn in 50% of ethanol, twice a day for 15 days. The sodium chloride was administrated into rabbits in the control group. MAIN OUTCOME MEASURES: The serum alkaline phosphatase (S-ALP), Ca2+, P3+, K+, Na+, Cl-, liberation and total calcium of the rabbit were measured at 15 and 30 days after drug administration, X-ray of the bones were performed at the fracture healing. RESULTS: Compared with the control group, the fracture healing of the experimental group was faster, and the serum examinations showed that the serum Ca2+ levels of the experimental group was significantly increased by Plumbago zeylanica Linn, there had significantly differences between prior to and after drug administration (P
ABSTRACT
To explore the anti-tumor effect of immunotherapy with recombinant protein vaccine based on FGFR-1 of chicken (cFR-1) in a mouse Meth A fibrosarcoma model, tumor volume and survival rate of the mice were observed at a 3-day interval. Microvessel density (MVD) was detected by immunohistochemistry. Auto-antibodies against self-FGFR-1 were detected by Western blotting and ELISA, respectively. The anti-FGFR-1 antibody-producing B cells (APBCs) were detected by enzyme-linked immunospot (ELISPOT) assay. Eighteen days after inoculation of tumor cells, the tumor volume was significantly smaller in cFR-1-immunized group than in mouse FGFR-1 (mFR-1) immunized group and normal saline (NS) control group (P<0.05), and the survival time was significantly longer in cFR-1-immunized group than in the control groups (P<0.01). MVD was significantly lower in cFR-1-immunized group than in mFR-1-immunized group and NS group (16.8+/-5.6 vs 64.6+/-1.8 and 59.6+/-8.7, P<0.01). Antibodies against self-FGFR-1 were found in mFR-1-immunized group, the major antibody subclasses were IgG1 and IgG2b. Compared with the two control groups, the numbers of APBCs in cFR-1-immunized group were significantly increased (P<0.01) These results demonstrated that the cFR-1-related anti-angiogenesis protein vaccine could induce the production of auto-antibodies against self-FGFR-1, which futher inhibit angiogenesis and growth of solid tumor.